■ 基本信息
啟動子: | Ac5 |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 昆蟲系列質粒；昆蟲游離質粒；昆蟲胞內質粒 |
質粒大小: | 6118bp |
質粒標簽: | N-EGFP，C-V5，C-6×His |
原核抗性: | Amp |
真核抗性: | 無 |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
表達宿主: | 果蠅細胞系S2 |
誘導方式: | 無需誘導，組成型表達 |
5'測序引物: | Ac5-F(ACACAAAGCCGCTCCATCAG) |
3'測序引物:
| BGH-R(TAGAAGGCACAGTCGAGG)
|
備注: | 非病毒 |
■ 質粒屬性
質粒宿主: | 昆蟲細胞 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: |
|
熒光標記: | 綠色
|
■ 質粒簡介
pAC5.1b-EGFP-2質粒是一個昆蟲細胞組成型表達載體，Ac5啟動子驅動EGFP標簽、目的基因、Myc標簽和6×HIS標簽一起融合表達，可以與篩選載體pCoHygro或者pCoBlast共轉染，建立穩(wěn)表達細胞系。
pAC5.1b-EGFP② is a 6kb expression vector designed for use with the Drosophila Constitutive Expression System available from Invitrogen. Upon transfection, the vector allows transient expression of your protein of interest in Drosophila cells. When cotransfected with the selection vector, pCoHygro or pCoBlast, pAC5.1b-EGFP allows selection of stable cell lines exhibiting constitutive expression of the protein of interest.
? The Drosophila actin 5C (Ac5) promoter for high-level, constitutive expression of the gene of interest in S2 cells (Chung and Keller, 1990).
? Multiple cloning site to facilitate cloning the gene of interest.
? C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for detection and purification of your protein of interest (if desired).
? Three reading frames to facilitate in-frame cloning with the C-terminal peptide.
? N-terminal EGFP tag for better detection.
? Ampicillin resistance gene for selection of transformants in E. coli.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK1035pAC5.1b-EGFP-2昆蟲胞內質粒.txt
質粒只保證關鍵序列正確，不保證表達效果。
